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OBSERVE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and in addition a number of the amino teams throughout the spot areas do not form bonds with the amino acids. WORD: This avoids the coupling of amino acids in the additional cycles with the membrane as a substitute of the rising peptide chain. Be certain that all the purification buffers are at four °C and carry out the protein purification within the chilly room to keep away from protein denaturation. |
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